complementary dnasの例文
- A complementary DNA encoding protease that carries out this cleavage has been cloned.
- The resulting cDNA ( complementary DNA ) encodes the extein along with the intein.
- Several applications have been developed that interrogate SNPs by hybridizing complementary DNA probes to the SNP site.
- The complementary DNA ( cDNA ) of human MMP7 was isolated in 1988 by Muller et al.
- The enzyme uses the viral RNA genome as a template for the synthesis of a complementary DNA copy.
- However, the Supreme Court also decided that complementary DNA can be patented because it is not naturally occurring.
- Traditionally DNA microarrays use complementary DNA or oligonucleotide probes to analyze messenger RNA ( mRNA ) from genes of interest.
- This method consists of hybridizing a labeled complementary DNA or RNA strand to a specific DNA or RNA in the tissue.
- First of all, the reverse transcriptase synthesizes viral DNA from viral RNA, and then from newly made complementary DNA strand.
- A sample of mRNA are first converted to complementary DNA ( cDNA ) using reverse transcriptase, which makes subsequent manipulations easier.
- The general steps to prepare a complementary DNA ( cDNA ) library for sequencing are described below, but often vary between platforms.
- Here, the two strands are separated and then each strand's complementary DNA sequence is recreated by an enzyme called DNA polymerase.
- In the case of HIV, reverse transcriptase is responsible for synthesizing a complementary DNA strand ( cDNA ) to the viral RNA genome.
- As noted above, the probe is either a labeled complementary DNA or, now most commonly, a complementary RNA ( riboprobe ).
- On the leading strand, DNA polymerase can make a complementary DNA strand without any difficulty because it goes from 5'to 3 '.
- Complementary DNA can be processed the same way as genomic DNA, allowing the expression levels of RNAs to be determined for the tissue selected.
- Moreover, analysis of their complementary DNA and ribosomal RNA revealed that the hybrids have very differential gene expressions compared to those in gray wolf controls.
- This complementary DNA, as it is called, can be sequenced in the form of short fragments that are called ESTs, for expressed sequence tags.
- During NHEJ, very short stretches of complementary DNA, 1 bp or more at a time, are hybridized together, and the overhangs are removed.
- The complementary DNA ( cDNA ) of the RPSA gene is formed by the assembly of seven exons, six of which correspond to the coding sequence.